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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in several tumors, which results in chromosome instability, and so could contribute to tumorigenesis. This protein was observed to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital part in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been explained. Two additional splice variants had been documented but detailed nucleotide sequence information and facts will not be nevertheless readily available.
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Blocking peptides are peptides that bind precisely on the target antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This guide outlines further more attainable optimization of mobile and immunoassay parameters to be certain the very best final results are...
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This guide outlines further more possible optimization of cellular and immunoassay parameters to make certain the best possible success are...
Antibodies certain to the blocking peptide no more bind towards the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin E belongs for the extremely conserved cyclin family members, whose users are characterised by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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